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. 2014 Jan 9;9(1):e85700. doi: 10.1371/journal.pone.0085700

Figure 2. Plasma nesfatin-1 concentrations in female rainbow trout recovering from spawning.

Figure 2

Mean (±SEM) plasma nesfatin-1 concentration over time in female rainbow trout (A: two-year-old; B: three-year-old) fed a control-ration or restricted-ration. When comparing treatment groups, mean values differ significantly (two-tailed t-test, p<0.05) at time-points marked “*” and marginally (two-tailed t-test, p<0.1) at time points marked “‡”. Within each figure, time-points sharing the same letter are not significantly different (Tukey’s HSD, p≥0.05). Latin letters (a, b, c, …) refer to control treatment fish; Greek letters (α, β, γ, …) refer to restricted ration fish. Figure A: Week 0, n = 42 per treatment; Week 5, n = 36 per treatment; Week 10, n = 30 per treatment; Week 15, n = 24 per treatment; Week 20, n = 18 per treatment; Week 25, n = 12 per treatment; Week 30, n = 6 per treatment. Figure B: Week 0, n = 25 per treatment; Week 4, n = 20 per treatment; Week 8, n = 15 per treatment; Week 12, n = 10 per treatment; Week 16, n = 5 per treatment.