Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cancer Res. 2013 Nov 6;74(1):10.1158/0008-5472.CAN-13-1536-T. doi: 10.1158/0008-5472.CAN-13-1536-T

Figure 1.

Figure 1

Expression of endogenous Bcl2 is correlated with decreased levels of RNR activity and intracellular dNTPs. A, expression levels of Bcl2, hRRM1 and hRRM2 were analyzed by Western blot. B-C, extracts from various human lung cancer cells were incubated with 14C-CDP. The generation of 14C-dCDP was analyzed by a phosphorImager on TLC. The relative abundance of 14C-dCDP and 14C-CDP was quantified with imageQuant software. RNR activity was calculated by a formula as 14C-dCDP/(14C-CDP+14C-dCDP). Error bars represent ±SD of three repeated determinations. D, intracellular levels of dNTPs in various human lung cancer cells were measured. Error bars represent ± SD of three repeated determinations.