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α1 internalization in rat α1-transfected cells. Internalization was measured by surface biotinylation as above in rat α1-expressing cells. The internalized human and rat α1 were discriminated using anti-YFP and anti-HA antibodies. Data are expressed as -fold increase in intracellular biotinylated α1. Means ± S.E. (error bars) of three experiments are depicted. *, p < 0.05.
