FIGURE 1.

SMILE decreases T7-mediated lipogenic gene expression. A and B, mouse primary hepatocytes and HepG2 cells were infected with Ad-SMILE or Ad-GFP and then exposed to T7. Whole cell extracts were isolated and analyzed using Western blot analysis with the indicated antibodies. Protein levels were normalized to α-tubulin level. Each data point represents mean ± S.E. C and D, quantitative PCR analysis was performed using total RNA from Ad-SMILE or Ad-GFP-infected mouse primary hepatocytes or HepG2 cells after T7 treatment. The Srebp-1c, Fas, Acc, SMILE, and β-actin genes were amplified using specific primers for Srebp-1c, Fas, Acc, SMILE, and β-actin. mRNA levels were normalized to β-actin expression. Results are representative of three independently performed experiments.