FIGURE 7.
SMILE improves T7-induced hepatic lipid accumulation. A, male 7-week-old C57BL6 mice were provided with a standard rodent diet. T0901317 (LXR agonist, 50 mg/kg body weight) or vehicle (1% methylcellulose and 1% Tween 80) were administered by oral gavage each day for 1 week. An aliquot of 0.5 × 109 plaque-forming units of GFP or SMILE adenovirus were delivered by tail vein injection on day 4 of oral gavage. Three days after injection, the mice were sacrificed and Oil Red-O staining was performed on the liver samples. B, liver triglyceride levels were analyzed from the mouse liver tissue as in A. C and D, liver cholesterol staining (C) and hepatic cholesterol levels (D) were analyzed as in A and B. E, Srebp-1c, Fas, and Acc mRNA levels in mouse liver were analyzed by real time quantitative RT-PCR. F, real time-quantitative RT-PCR analysis of hepatic Srebp-1c, Fas, and Acc in adenovirus GFP or SMILE injected mice that were fed chow or HFD for 12 weeks. All data were normalized to those of β-actin and ribosomal L32 expression. Data in B and D–F are represented as mean ± S.D.