FIGURE 4.

CUGBP1-eIF2 complexes increase translation of C/EBPβ-LIP in S193D mice. A, Western blotting assay shows expression of CUGBP1 and eIF2α in WT and in S193D livers after CCl₄ treatment. Bottom part (CUGBP1 IP) shows Co-IP studies. B, levels of CUGBP1 protein were calculated as ratios to β-actin. Bottom image shows expression of CUGBP1 mRNA after treatments with CCl₄. C, amounts of CUGBP1-eIF2α complexes were calculated as ratios of eIF2α in CUGBP1 IP to signals of total eIF2α. D, expression of cyclin D3 and cdk4 after CCl₄ treatment was examined by Western blotting. E, examination of CUGBP1 phosphorylation by two-dimensional gel electrophoresis-Western blotting. Positions of highly phosphorylated forms of CUGBP1 are shown by red arrows. F, association of CUGBP1 and eIF2α with C/EBPβ-mRNA after CCl₄ treatment. CUGBP1 and eIF2α were immunoprecipitated, RNA was isolated and the presence of C/EBPβ mRNA was determined by RT-PCR.