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Liver proliferation and liver fibrosis are increased in young S193D mice after chronic treatments with CCl4. A, animals were treated by CCl4 for 4 weeks as described under “Experimental Procedures.” Livers of WT and S193D mice were stained with H&E and with antibodies to BrdU. Reproducible pictures are shown. B, levels of ALT and AST in blood and calculations of BrdU-positive hepatocytes are shown in the bar graph pictures. C, livers were stained with α-SMA and Sirius Red.
