FIGURE 1.

ITC isotherms for the binding of bNAb 2F5 to its core and functional epitope peptides. A and B, direct ITC isotherms for the binding of 2F5 (in both the IgG and Fab forms) to the E7S and N16N peptides, respectively. C, ITC displacement isotherm for the binding of 2F5 IgG to N16N using E7S as the weak ligand. In all panels, the symbols represent the experimental heats measured for each peptide injection normalized per mole of injected peptide. The curves correspond to the best fittings using a model of n identical sites for the direct titrations and a binding competition model for the displacement experiment.