TABLE 3.

Relative glycosylation turnover rates of different LLO donor substrates and PglB Tyr²⁹³ mutants

The relative turnover (-fold reduction) refers to the activity of an individual reaction compared with the reaction of WT PglB and LLO with the heptasaccharide structure. Activities were determined using fluorescently labeled acceptor peptide, and individual turnover rates were derived from three independent experiments. Red hexagon, di-N-acetylbacillosamine (diNAcBac); yellow square, N-acetylgalactosamine; blue circle, glucose.