TABLE 1.
Primers and siRNAs used in this study
| Primer/siRNA | Sequence |
|---|---|
| Primers for plasmid construction | |
| FLAG-HER2-F | ttaagcttgccaccatgATGGAGCTGGCGGCCTTGTG |
| FLAG-HER2-R | ttgcggccgcCACTGGCACGTCCAGACCCA |
| HER2-ECD-F | ttaagcttATGGAGCTGGCGGCCTTGTG |
| HER2-ECD-R | ttgcggccgcGTCCACACAGGAGTGGGT |
| HER2-ICD-F | ttaagcttAAGCGACGGCAGCAGAA |
| HER2-ICD-R | ttgcggccgcCACTGGCACGTCCAGAC |
| HER2-ICD-1-F | ttaagcttgccaccatgAAGCGACGGCAGCAGAAGAT |
| HER2-ICD-1-R | ttgcggccgcGTCTGTAATTTTGACATGGT |
| HER2-ICD-2-F | ttaagcttgccaccatgGAAGCATACGTGATGGCTGG |
| HER2-ICD-2-R | ttgcggccgcTTTGACCAT GATCATGTAG |
| HER2-ICD-3-F | ttaagcttgccaccatgGTCAAATGTTGGATGATT |
| HER2-ICD-3-R | ttgcggccgcGGGCGAAGGGGGCTGGGGCCG |
| HER2-ICD-4-F | ttaagcttgccaccatgCGAGAGGGCCCTCTG |
| HER2-ICD-4-R | ttgcggccgcCACTGGCACGTCCAGACCCA |
| GST-S100A14-F | ttggatccATGGGACAGTGTCGGTCAGC |
| GST-S100A14-R | ataagatgcggccgcTCAGTGCCCCCGGACAGGCC |
| S100A14-Δ1-F | ttggatccATGGGACAGTGTCGGTCAGC |
| S100A14-Δ1-R | ataagatgcggccgcTCAGAAACTCCTGAACTCCAGTT |
| S100A14-Δ2-F | ttggatccCCTTCTGAGCTACGGGACCTGG |
| S100A14-Δ2-R | ataagatgcggccgcTCAGTGCCCCCGGACAGGCC |
| S100A14-Δ3-F | TTTCACCAGTACCCTTCTGAGCTA |
| S100A14-Δ3-R | TAGCTCAGAAGGGTACTGGTGAAA |
| S100A14-Δ4-F | ATTGCCAACCTGAGGAGTTTCTGG |
| S100A14-Δ4-R | CCAGAAACTCCTCAGGTTGGCAAT |
| D76A-F | CAGCTGCAATGCCTCTAAACTGG |
| D76A-R | CCAGTTTAGAGGCATTGCAGCTG |
| F81A-F | TAAACTGGAGGCCAGGAGTTTCT |
| F81A-R | AGAAACTCCTGGCCTCCAGT TTA |
| S83R-F | GGAGTTCAGGAGATTCTGGGAGC |
| S83R-R | GCTCCCAGAATCTCCTGAACTCC |
| F81AS83R-F | GGAGGCCAGGAGATTCTGGGAGC |
| F81AS83R-R | GCTCCCAGAATCTCCTGGCCTCC |
| siRNAs | |
| siControla | |
| siS100A14b | 1) GCUGACCCCUUCUGAGCUA |
| 2) CAGAGGAUGCUCAGGAAUU | |
| 3) GAGAACUCCCUCUGGAAUU | |
| 4) UCAAGAACUUUCACCAGUA | |
a Dharmacon ONTARGETplus non-targeting siRNA (D-001810-10-20).
b Dharmacon Non-TARGETplus siRNA.