FIGURE 1.

The LRRK2 Roc GTPase domain binds TUBB and TUBB4. A, full-length myc-tagged LRRK2 interacts with full-length FLAG-tagged TUBB and TUBB4 in co-immunoprecipitation experiments (CL, cell lysate; IP, = anti-FLAG immunoprecipitates; transfections as indicated). B, the LRRK2 RocCOR domain and the C termini of interacting β-tubulin isoforms were sufficient for interaction. C, semiquantitative YTH experiments show interaction with TUBB and TUBB4 to require the LRRK2 Roc but not COR domain. D, semiquantitative lacZ freeze-fracture assays show that the LRRK2 guanine nucleotide-binding mutant K1347A abolishes LRRK2 interactions with TUBB and TUBB4 but not LRRK2 RocCOR dimerization.