FIGURE 7.

Pep2-8 solution structure by NMR and from the Pep2-8·PCSK9-ΔCRD complex x-ray structure. Peptide backbone shown with Cα atoms only. The aromatic amino acid side chains Phe³, Trp⁶, and Tyr⁹ are shown. Side chain atoms of Trp¹² are omitted for clarity. N, N terminus; C, C terminus. A, the 20 lowest energy NMR structures describe a consistent peptide conformation (pairwise root mean square deviation for backbone atoms 0.36 ± 0.13 Å (Table 3)). The lowest energy NMR structure is in red, and the next 19 lowest energy models from NMR are shown in black. B, the Pep2-8 structure from the Pep2-8·PCSK9-ΔCRD x-ray structure (green) is essentially the same as the lowest energy solution structure from NMR (red).