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. 2013 Nov 14;289(2):956–967. doi: 10.1074/jbc.M113.515445

FIGURE 7.

FIGURE 7.

Exogenously added and internalized Tau propagates from cell to cell. A, the culture medium containing either released K18*-488 or released hTau40*-488 was transferred onto fresh cells and incubated for an additional 72 h. Re-uptaken K18*-488 displays a fluorescence lifetime of 2637 ± 40 ps (ii), and i displays the corresponding confocal image. Re-uptaken hTau40*-488 displays a fluorescence lifetime of 2575 ± 51 ps (iv), and iii displays the corresponding confocal image. Scale bars = 10 μm. B, corresponding bar diagram representing the mean fluorescence lifetimes observed for re-uptaken Tau. The dotted lines represent the mean fluorescence lifetime of monomeric and endocytosed K18*-488. Error bars represent S.D. One-way analysis of variance statistical analysis was performed for K18* (F (2, 72) = 2995, p < 0.0001) and hTau40* (F (2, 46) = 1120, p < 0.0001). ****, p < 0.0001.