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. 2013 Nov 25;289(2):987–999. doi: 10.1074/jbc.M113.525428

FIGURE 2.

FIGURE 2.

A, SDS-PAGE analysis of the purified LIL3-GGR complexes. M, molecular weight marker; 1, protein profile of purified WT extracts as a negative control; 2, protein profile of the purified extracts from the LIL3-FLAG-expressing plant. B, BN-PAGE analysis of the purified LIL3-GGR complexes. Two molecular weight markers (NativeMark from Invitrogen and the HMW calibration kit (HMW Cal Kit) from GE Healthcare) were run for comparison. 1, protein profile of purified WT extracts as a negative control; 2, protein profile of the purified extracts from the LIL3-FLAG-expressing plant. C, two-dimensional BN-PAGE/SDS-PAGE analysis of the purified LIL3-GGR complexes. For BN-PAGE analysis, the HMW calibration kit was used for estimation of the molecular weights, and gels were stained with a silver staining kit (Thermo Fisher Scientific Inc.).