Figure 4.

Induction of STAT3 transcriptional activity by G-CSF. (A) Induction of STAT3 DNA binding by G-CSF. Nuclear extracts were prepared from OVCA429 cells either untreated (Ctrl) or stimulated by G-CSF or IL-6 alone (No Inh) or in combination with inhibitors for STAT3 (S3I) or JAK2 (J2I), as indicated. (B–E) Expression of STAT3-responsive genes by G-CSF. Cells either untreated or treated with G-CSF or IL-6 were analysed by semi-quantitative RT–PCR (B), with the fold change determined by subsequent real-time RT–PCR (C, *P<0.05 compared with the control). Expression of the encoded proteins in these cells was determined by western blot analysis as indicated (D). For BCL2, this analysis was repeated in the presence of inhibitors for JAK2 (J2I) and STAT3 (S3I) (E).