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. 2013 Nov 12;110(1):133–145. doi: 10.1038/bjc.2013.673

Figure 5.

Figure 5

Interaction between chemotherapeutic agents and G-CSF/G-CSFR. (A–E) The effects of the chemotherapeutic agents cisplatin and paclitaxel on the expression of G-CSF/G-CSFR. The impact of 5-day chemotherapy treatment on the indicated ovarian cancer cell lines was determined by RT–PCR for G-CSFR and G-CSF in response to cisplatin (A) or paclitaxel (D), analysis of cell supernatants using ELISA for G-CSF in response to cisplatin (B), or FACS analysis for G-CSFR expression in response to cisplatin (C) or paclitaxel (E) (red line: untreated, blue line: chemotherapy agent) or isotype control (black line). (F) Chemotherapeutic agents activate NFκB. Cell lysates were prepared from OVCA433 cells treated with cisplatin (Cis) or paclitaxel (Pac) as indicated and subjected to western blot analysis with anti-phospho-NFκB, anti-NFκB or anti-GAPDH.