Figure 6.

Induction of HSP70 and NQO1 (A and B) and inhibition of cell proliferation (C and D) by sulphoxythiocarbamates. (A) WT (2.1 × 10⁵ per well) or (B) HSF1-KO (2.5 × 10⁵ per well) MEFs in six-well plates were exposed to vehicle (0.1% acetonitrile) or increasing concentrations of each sulphoxythiocarbamate for 4 h. Cells were lysed in RIPA buffer 20 h later, aliquots from cell lysates were resolved by SDS–PAGE, transferred to immobilon-P, and probed with antibodies against HSP70 and NQO1. GAPDH was used as a loading control. The data are representative from two independent experiments. (C and D) MEFs (10⁴ per well, in 96-well plates) were treated with vehicle (0.1% acetonitrile) or increasing concentrations of sulphoxythiocarbamates of the K-series (C) or the S-series (D) for 24 h. Cell proliferation was assessed using the Alamar Blue fluorometric assay. Data represent means±s.d. from six replicate wells. *P<0.05 relative to vehicle-treated cells.