Fig. 4. Identification of de novo transposition events in αβ neurons.

(A) Venn diagram of transposon insertions identified in this study. Direct genome sequencing identified 215 insertion sites in αβ neuron DNA and 200 in DNA from other brain cells that were not present in embryo DNA from genetically identical sibling flies. (B) Chromosomal distribution of new transposon insertions in αβ neurons. Bar height indicates number of insertions in each location. piRNA clusters are shown. (C) The proportion of new insertions found for each transposon class in αβ neurons. (D) Proportion of insertions per transposon class in the inherited fly genome from our sequencing of embryo DNA. (E) Distribution of transposons in the annotated genome significantly differs from de novo insertions in αβ neurons, the rest of brain and ovary DNA with respect to neighboring genes (p-value <2.2E-16 for αβ neurons, the rest of brain and ovary; χ² test).