Figure 2. Decreased cell survival is directly correlated with decreased NF-κB activity following treatment with a panel of NF-κB/IKK inhibitors.

293^NF-κB reporter cell line was utilized to measure relative levels of NF-κB activation 3 hours after TNFα stimulation (10 ng/ml) at varying concentrations of the respective inhibitors (black lines). FSDC (grey lines) were treated with the same dose of the five NF-κB inhibitors and percent survival was determined by MTT assay after 24 hr incubation. FSDC, unlike the 293^NF-κB, were not stimulated with exogenous TNFα. Data are representative of 5 independent experiments in triplicate.