Figure 5.

The boundary of RALDH-2 and RALDH-3 expression by IHC matches the boundary defined by OCAM/mamFasII. A: IHC analysis using antibodies against both OCAM/mamFasII (green), and RALDH-2 (red). The boundary marked by RALDH-2 can be placed by the sharp decline in red label of both the epithelium and by the abrupt fall-off in the staining of the cells in the superficial aspects of the lamina propria. The boundary marked by OCAM/mamFasII is marked best by the abrupt decline in the staining of the small nerve fascicles (examples indicated by the single arrows) immediately subjacent to the basal lamina, as shown previously (Schwob and Gottlieb, 1986). The boundary for each expression domain corresponds closely and that common boundary is marked by the double arrows. A1,A2: The insets provide a higher magnification view of the differences in the superficial lamina propria. B: Likewise, OCAM/mamFasII (green) and RALDH-3 (red) also share a common boundary as defined above (double arrows). Again, note the relation of RALDH-3 expression to the heavily labeled OCAM/mamFasII (+) nerve fascicles that are evident as they exit the epithelium (B1 arrows). A magenta-green version of this Figure may be found online as Supporting Figure 5. Scale bars = 50 μm in A,B; 20 μm in insets.