FIG. 4.
Lin−/Sca-1+/c-kit+ (LSK) cells from aging KO mice have altered levels of reactive oxygen species (ROS) (DCFDA staining), Ki-67, p16Ink4a and γ-H2A.X. Lin- cells from 1.5 year old mice (n=5–8) were isolated and used for separate experiments for each endpoint as described in Material and Methods. Stained Lin- cells were gated for LSK cells as shown in Supplementary Fig. S6, and mean fluorescence intensity (MFI) profiles of individual antibody staining were generated (A–D). MFI data for LSK cells from separate groups were generated and presented as mean±S.D (A–D). *Values significantly different from control (P<0.05).