FIG. 2.

Cited2 KO HSCs display decreased glutathione content and elevated cellular levels of reactive oxygen species. (A) Representative plot of monochlorobimane staining on LT-HSCs, ST-HSCs, and WBM. (B) Quantitative analysis of monochlorobimane MFI (mean±SD, n=3). (C) Representative plot of dichlorodihydrofluorescein diacetate (DCFDA) staining on LT-HSCs and ST-HSCs. (D) Quantitative analysis of fold changes in DCFDA staining (mean±SD, n=6). The fold changes were calculated based upon DCFDA MFI of KO relative to that of WT HSCs. Asterisks are explained in Materials and Methods Statistics section.