FIG. 3.

Loss of Cited2 decreases glycolysis in HSCs. (A) Representative plot of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) fluorescence on LT-HSCs, ST-HSCs, and WBM. Gray-filled histogram indicates control without 2-NBDG. (B) Quantitative analysis of 2-NBDG MFI on cells (mean±SD, n=3). (C) Relative intracellular adenosine-triphosphate (ATP) contents in various cell populations. The ATP level in WT LT-HSCs was set as one, and the ATP levels in other cell populations were presented as the relative values to that of WT LT-HSCs (mean±SD, n=6). (D) Glucose-derived ¹³C-lactate production per 1×10⁴ cells (mean±SD, n=4). (E) Representative plot of cellular NADH: flow cytometry profiles of cells before (upper panel) and after (lower panel) treatment with antimycin A (AMA). (F) Percentage of cells that display increased nicotinamide adenine dinucleotide+hydrogen (NADH) fluorescence in response to AMA treatment (mean±SD, n=6). Asterisks are explained in Materials and Methods Statistics section.