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. 2013 Oct 1;23(2):83–94. doi: 10.1089/scd.2013.0370

FIG. 6.

FIG. 6.

Cited2 deletion leads to increased phosphorylation of FoxOs in HSCs. (A) Representative plot of p-FoxO1 (Ser256) staining. (B) Quantitative analysis of p-FoxO1 MFI on cells. (C) Representative plot of p-FoxO3a (Ser253) staining. (D) Quantitative analysis of p-FoxO3a MFI on cells. (E) Relative expression of genes determined by real-time quantitative PCR. Fresh bone marrow cells were cultured at 37°C under normoxia for 90 min in DMEM supplemented with 15% FBS, 50 ng/mL rmSCF, 20 ng/mL rmIL-3, and 50 ng/mL rhIL-6, with or without 10 μM PI3K/Akt inhibitor LY294002. LT-HSCs were then sorted for RNA extraction and gene expression analysis. The results are expressed as mean±SD (n=4–6). Asterisks are explained in Materials and Methods Statistics section.