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. 2014 Jan 1;34(1):16–21. doi: 10.1089/jir.2013.0038

FIG. 1.

FIG. 1.

Hepatitis C virus (HCV) NS5A suppresses IRF-7-mediated interferon (IFN)-α14 promoter activity. (A) Immortalized human hepatocytes (IHH) were cotransfected with human IFN-α4, IFN-α7, or IFN-α14 promoter with the Luc reporter and IRF-7 plasmid constructs. Cell extracts were prepared 48 h post-transfection and the luciferase activity was measured. (B) IHH were cotransfected with human IFN-α14-luc reporter plasmid, IRF-7, and HCV genomic region encoding specific protein(s). Cell extracts were prepared for luciferase assay 48 h post-transfection. (C) IHH were cotransfected with human IFN-α14-luc reporter plasmid, IRF-7 construct, and/or HCV NS5A. Cell extracts were prepared for luciferase assay 48 h post-transfection. Results presented are mean with standard error from 3 independent experiments. Empty vector DNA was used as a negative control and the mean basal value was arbitrarily set at 1 for all luciferase assays.