FIG. 1.

Phenotypic characterization of rat pES cells. (a) Outgrowth of a parthenogenetic blastocyst on a MEF feeder layer after 5 days of culture. (b) Colony formation of pES cells on MEFs at passage 6. (c) Alkaline phosphatase-positive colonies of rpESWIv2iF-5 at passage 7. (d) X-chromosome monosomy of rpESWIv2iF-5 cells at passage 8 by G-band staining. Scale bars: (a, b) 100 μm and (c) 200 μm. pES, parthenogenetic blastocyst-derived embryonic stem; MEF, mouse embryonic fibroblast. Color images available online at www.liebertpub.com/scd