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. 2013 Sep 6;23(2):107–114. doi: 10.1089/scd.2013.0200

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 2. — RT-PCR analysis. (a) Three rat pES cell lines (rpESWIv2iF-2, rpESWIv2iF-5, and rpESWIv2iF-10), a rat ES cell line (rESWIv3i-1), and rat embryonic fibroblasts at E14.5 (rEF; negative control for stem cell markers). The three pES cell lines expressed stem cell marker genes, Oct-4, rNanog, Fgf-4, and Rex-1. (b) Expression of Cdx2, a trophectoderm-specific marker gene. Colon from day-2 rat offspring and rEF were used as positive and negative controls, respectively. RT-PCR, reverse transcriptase-polymerase chain reaction.