FIG. 5.

Detection of thyroglobulin in the mouse thyroid by immunogold electron microscopy. After wash with phosphate-buffered saline–bovine serum albumin (PBS-BSA 1%), ultrathin sections (0.1 μm) were incubated overnight with a rabbit polyclonal anti-thyroglobulin antibody (1/300, DAKO). Sections were then rinsed and incubated for 30 minutes with a 12-nm colloidal gold affinity pure goat anti-rabbit IgG (Jackson, 111-205-144, lot no. 71647). Sections were postfixed with 2.5% glutaraldehyde for 5 minutes and counterstained. They were examined with a Zeiss 109 transmission electron microscope. (A) Negative control obtained by omission of primary antibody. (B) Thyroglobulin was detected as small gold particles in the colloid limited by flat epithelial cells. Reproduced with permission from Senou et al. (20).