FIG. 8.

Supply and metabolism of thyroid hormones affect negatively and positively T₃-regulated genes in the brain. To construct this figure, the authors used individual reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) data from T₃-regulated genes to calculate the fold change relative to the wild-type (WT) values, and plotted the Log₂FC (fold change) to make the results quantitatively comparable. The data were represented in a box-and-whiskers (5%–95%) plot. Statistical significance between each group and the WT was calculated by one-way ANOVA. For the positive genes, F_5,537=272, p<0.0001. For the negative genes, F_5,400=145, p<0.0001. *p<0.05; **p<0.01; ***p<0.001. Reproduced with permission from Hernandez et al. (492).