Table 1.

Organization of the Task Force's Recommendations

Location key	Sections and subsections	Page
[A]	Assessing the Thyroid Gland	91
[A.1]	Structure–function relationships	91
	Recommendation 1	91
[A.2]	Thyroid iodide kinetics	93
	Recommendation 2	94
	Recommendation 3	95
[A.3]	Thyroid imaging	95
	Recommendation 4	95
[B]	Assessing Circulating and Tissue Thyroid Hormone Levels	97
[B.1]	Serum	97
	Recommendation 5	98
	Recommendation 6	99
	Recommendation 7	100
[B.2]	Tissue	100
	Recommendation 8	100
[B.3]	Sources of tissue T3 and TR saturation	100
	Recommendation 9	101
[C]	Assessing Thyroid Hormone Transport Into Cells	101
[C.1]	Thyroid hormone transport in vitro	102
	Recommendation 10	102
	Recommendation 11	103
[C.2]	Thyroid hormone transport in vivo	103
	Recommendation 12	103
[D]	Assessing Thyroid Hormone Deiodination	104
[D.1]	Identification, expression, and quantification of deiodinases	104
	Recommendation 13	104
	Recommendation 14	105
[D.2]	Deiodination in intact cells	106
	Recommendation 15	106
[D.3]	Deiodination in perfused organs	106
	Recommendation 16	106
[D.4]	Deiodination in whole animals	107
	Recommendation 17	107
[D.5]	Non-deiodination pathways of thyroid hormone metabolism	108
	Recommendation 18	109
[E]	Inducing Hypothyroidism and Thyroid Hormone Replacement	109
[E.1]	Hypothyroidism in animals	109
	Recommendation 19	109
	Recommendation 20	110
	Recommendation 21	111
	Recommendation 22	111
	Recommendation 23	112
[E.2]	Thyroid hormone replacement in animals	113
	Recommendation 24	113
[E.3]	Hypothyroidism in cultured cells	114
	Recommendation 25	114
[F]	Increasing Thyroid Hormone Signaling	114
[F.1]	Thyrotoxicosis in animals	114
	Recommendation 26	115
	Recommendation 27	115
[F.2]	Thyrotoxicosis in cultured cells	115
	Recommendation 28	115
[F.3]	Use of thyroid hormone analogues	116
	Recommendation 29	116
[G]	Iodine Deficiency and Maternal–Fetal Transfer of Thyroid Hormone	117
[G.1]	Iodine deficiency in rodents	117
	Recommendation 30	117
	Recommendation 31	118
	Recommendation 32	118
[G.2]	Placental transfer of thyroid hormone	118
	Recommendation 33	118
[H]	Models of Nonthyroidal Illness	118
	Recommendation 34	119
	Recommendation 35	119
[I]	Assessing Thyroid Hormone Signaling at Tissue and Cellular Levels	119
[I.1]	Gene expression as a marker of thyroid hormone status	120
	Recommendation 36	120
[I.2]	PCR analysis of mRNA expression levels	120
	Recommendation 37	120
[I.3]	Genome-wide analysis of thyroid hormone-responsive mRNA	122
	Recommendation 38	122
[I.4]	Mechanisms of gene regulation by thyroid hormone	122
	Recommendation 39	122
	Recommendation 40	123
[I.5]	Mouse models for indicating thyroid hormone and TR signaling in tissues	123
	Recommendation 41	124
[J]	Assessing Thyroid Hormone Signaling by Way of Systemic Biological Parameters	124
[J.1]	Central nervous system	125
	Recommendation 42	126
	Recommendation 43	126
	Recommendation 44	127
	Recommendation 45	127
	Recommendation 46	127
	Recommendation 47	128
	Recommendation 48	128
[J.2]	Heart and cardiovascular system	129
	Recommendation 49	129
	Recommendation 50	129
	Recommendation 51	130
	Recommendation 52	130
	Recommendation 53	131
	Recommendation 54	132
[J.3]	Intermediary metabolism and energy homeostasis	132
	Recommendation 55	132
	Recommendation 56	135
	Recommendation 57	135
	Recommendation 58	136
	Recommendation 59	137
[J.4]	Skeletal muscle	137
	Recommendation 60	138
	Recommendation 61	138
	Recommendation 62	138
	Recommendation 63	139
	Recommendation 64	139
	Recommendation 65	139
[J.5]	Skeleton	140
	Recommendation 66	140
	Recommendation 67	140
	Recommendation 68	140
	Recommendation 69	140
	Recommendation 70	142

T₃, 3,3′,5-triiodothyronine; TR, thyroid hormone receptor; PCR, polymerase chain reaction.