Table 2.
Free Fractions of T4 and T3 (×100 and Expressed as Percentage) for the Common Media Types Containing Different Percentages of Bovine Serum Albumin/Serum
| Media type | T3 (%) | T4 (%) | Reference |
|---|---|---|---|
| BSAa | |||
| 4% |
0.56 |
0.09 |
(648) |
| 1% |
2.18–2.3 |
0.29–0.32 |
(745,746) |
| 0.5% |
3.45–3.78 |
0.41 |
(747–749) |
| 0.1% |
— |
3.39–3.6 |
(747,748) |
| Bovine serum | |||
| 10%b |
4 |
0.45 |
(316) |
| 10% (stripped)c |
1.5–4 |
0.8 |
(750,751) |
| 10% (Tx) | 0.4–2.0 | (281) | |
a
Direct measurements by equilibrium dialysis.
b
Direct measurement by ultrafiltration.
c
Calculated based on total and free hormone concentrations (equilibrium dialysis).
T4, thyroxine; BSA, bovine serum albumin; Tx, thyroidectomized.