FIG. 8.

Stimulation of mitoK⁺_ATP with diazoxide increases mitochondrial and cytoplasmic O₂^•−. (A) Mitochondrial O₂^•− measured with MitoSOX (2 μM) after the stimulation of HAEC with AngII or diazoxide (100 nM) plus PKCɛ inhibitor (1 μM). (B) Cytoplasm O₂^•− measured with DHE (10 μM) in HAEC treated with AngII (200 nM, 4 h) or diazoxide (100 nM, 10 min) plus PKCɛ inhibitor (1 μM). Figure show typical images from confocal microscopy studies obtained in five independent experiments. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars