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. 2014 Jan 10;20(2):295–307. doi: 10.1089/ars.2012.4606

FIG. 2.

FIG. 2.

Representative total-ion chromatograms of trypsin-digested mitochondrial proteins before and after immunoaffinity capture. Solubilized C6 mitochondria untreated or treated with 8-nitro-cGMP (100 μM) for 12 h were subjected to trypsin digestion, followed by LC-MS/MS with or without immunoaffinity capture. 8-Nitro-cGMP, 8-nitroguanosine 3′,5′-cyclic monophosphate.