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2D-gel electrophoretic images of S-guanylation Western blotting and silver staining of mitochondrial proteins. (A) Mitochondrial proteins solubilized in RIPA buffer were treated with 8-nitro-cGMP (200 μM) for 3 h. (B) Intact mitochondria were treated with 8-nitro-cGMP (200 μM) for 3 h. Spots in silver-stained gels were excised and subjected to in-gel digestion, followed by LC-MS/MS.
