FIG. 7.

Effects of MAO activation on mitochondrial ROS generation. (A) Images showing cell transfected with mitochondria-targeted HyPer (HyPer_mito), stained with mitochondrial dye Mitotracker Red and their overlap. Graph on the left shows HyPer_mito fluorescence ratio change with time after DA addition without (blue line) or with (red line) pargyline (parg). Graph on the right shows mitochondrial ROS production determined by mitochondria-targeted Hyper 30 min after DA addition. (B) Adult cardiomyocytes isolated from WT and MAO-B^−/− mice stained with Mitotracker Red CM-H₂XRos (MTR) before and after the addition of DA (left panel). MTR fluorescence intensity ratio is quantified on the right. WT: white bars, MAO-B^−/−: black bars. *p<0.05 versus control, ^†p<0.05 versus DA, ^‡p<0.001 versus WT vehicle, ^§p<0.005 WT DA versus MAO-B^−/− DA. CT, control; ROS, reactive oxygen species; WT, wild type; F/F₀, fluorescence intensity relative to resting fluorescence. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars