Skip to main content
. Author manuscript; available in PMC: 2014 Nov 27.
Published in final edited form as: Cell Rep. 2013 Nov 14;5(4):10.1016/j.celrep.2013.10.022. doi: 10.1016/j.celrep.2013.10.022

Figure 3. Constitutively-active PI3K restores B cell development in Baff−/− mice.

Figure 3

(A) Flow cytometry of B220+ splenic cells from Pten+/+Baff+/+Cd19Cre, PtenL/LBaff+/+Cd19Cre, Pten+/+Baff−/−Cd19Cre, and PtenL/LBaff−/−Cd19Cre mice. Data are representative of n>8 mice per group. (B) Absolute numbers of splenocytes and splenic B220+ B cells (top panel), and splenic B cell subsets (bottom panel). Shown is n=7 mice per group from n=5 experiments; small horizontal lines indicate mean. (C) Expression of CD21/35 on B220+-gated IgMloIgDhi splenic B cells from Pten+/+Baff+/+Cd19Cre, PtenL/LBaff+/+Cd19Cre, Pten+/+Baff−/−Cd19Cre, and PtenL/LBaff−/−Cd19Cre mice. MFI=mean fluorescence intensity. (D) ELISA of nitrophenol-specific IgM (top) or IgG (bottom) in the sera of Pten+/+Baff+/+Cd19Cre, PtenL/LBaff+/+Cd19Cre, Pten+/+Baff−/−Cd19Cre, and PtenL/LBaff−/−Cd19Cre mice prior to immunization (day 0), and 7 or 14 days post-immunization with 100 μg NP-KLH in alum. (E) Flow cytometric analysis of splenic GC B cells (B220+ gated) from immunized mice (top). Graph summarizes the percent of B220+GL7+Fas+ B cells 14 days post-immunization (bottom).