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. Author manuscript; available in PMC: 2014 Nov 27.
Published in final edited form as: Cell Rep. 2013 Nov 14;5(4):10.1016/j.celrep.2013.10.022. doi: 10.1016/j.celrep.2013.10.022

Figure 4. Upregulation of activation markers and proliferation are restored in Baff−/− B cells lacking Pten.

Figure 4

(A) Flow cytometric analysis of CD69 expression on Pten+/+Baff+/+Cd19Cre, PtenL/LBaff+/+Cd19Cre, Pten+/+Baff−/−Cd19Cre, and PtenL/LBaff−/−Cd19Cre B cells following stimulation with indicated mitogens. (B) As in (A), expression of CD86. (C) Purified splenic B cells from Pten+/+Baff+/+Cd19Cre, PtenL/LBaff+/+Cd19Cre, Pten+/+Baff−/−Cd19Cre, and PtenL/LBaff−/−Cd19Cre mice were stimulated as indicated. Proliferation was determined at 48 hours by 3H-thymidine incorporation. All assays were conducted in triplicate and SDs are shown as error bars. Data are representative of 3 independent experiments with n=2 mice per group per experiment. (D) Pten+/+Cd19Cre or PtenL/LCd19Cre mature lymph node B cells were left untreated or were cultured in the presence of BAFF, and cell viability was assessed by Annexin V (AnnV) and propidium iodide (PI) staining. Graph shows the percent of live (AnnV−PI−) cells at each time point. Data are representative of n=3 experiments with 5 mice/group total.