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. Author manuscript; available in PMC: 2014 Nov 27.
Published in final edited form as: Cell Rep. 2013 Nov 14;5(4):10.1016/j.celrep.2013.10.022. doi: 10.1016/j.celrep.2013.10.022

Figure 5. Lymphoma development in PtenL/LShipL/LCd19Cre mice occurs in a BAFF-independent manner.

Figure 5

(A) Flow cytometric analysis of B220+-gated splenic cells from Pten+/+Ship+/+Baff+/+Cd19Cre, Pten+/+Ship+/+Baff−/−Cd19Cre, PtenL/LShipL/LBaff+/+Cd19Cre, and PtenL/LShipL/LBaff−/−Cd19Cre mice. Data are representative of 2 independent experiments with n≥2–3 mice per group. (B) Absolute numbers of splenocytes and splenic B cells (n=3 mice per group; small horizontal lines indicate mean). (C) Kaplan-Meier survival curve of Pten+/+Ship+/+Baff+/+Cd19Cre (n=7), PtenL/LShipL/LBaff+/+Cd19Cre (n=6), and PtenL/LShipL/LBaff−/−Cd19Cre (n=9) mice. (D) Expansion of B220−/lowCD19+ lymphoma B cells in peripheral blood of Pten+/+Ship+/+Baff+/+Cd19Cre, PtenL/LShipL/LBaff+/+Cd19Cre, and PtenL/LShipL/LBaff−/−Cd19Cre mice determined by flow cytometry at indicated time points. Data shown are from two representative animals for each group. The PtenL/LShipL/LBaff+/+Cd19Cre animal shown in bottom row died before 9 months of age.