Fig. 5. Electrophoretic mobility and crosslinking of AS-treated apoA-I in a lipid-bound state. Lane 1, fructose + apoA-I; lane 2, aspartame + apoA-I; lane 3, acesulfame K + apoA-I; lane 4, saccharin + apoA-I; lane C, PBS + apoA-I. (A) Each POPC-rHDL migrated on an 8-25% native gradient gel electrophoresis without denaturation. Lane M, high-range molecular weight marker (GE Healthcare). (B) BS3-crosslinked POPC-rHDL was electrophoresed on 8-25% SDS-PAGGE. Lane M, molecular weight marker (GE Healthcare).
