Fig. 1. Effect of I. obliquus on ROS scavenging and inhibition of lipid peroxidation. Confluent cells were incubated with I. obliquus extract for 4 h prior to exposure to 1 mM hydrogen peroxide for 2 h. (A) Intracellular ROS scavenging activity of I. obliquus. Intracellular ROS generation was detected using the DCF-DA method. (B) Fluorescence microscopic images of cells stained for ROS. Representative images illustrate the increased green fluorescence intensity of DCF produced by ROS in hydrogen peroxide-treated cells compared to control as well as the lowered fluorescence intensity in hydrogen peroxide- treated cells in the presence of I. obliquus extract (25 μg/ml). Magnification: 400- fold. (C) Lipid peroxidation was detected by measuring the amount of TBARS. Data (A and C) represent the mean ± SE of three independent experiments. Significant differences were compared with the control at *p < 0.05 by Student’s t-test.