Fig. 2. Identifying NLS in MCRS2. Sequence between amino acids 57 and 73 was mutated by substituting these amino acids for alanines. At 18 h after transfection, proteins in the cells were stained with antifibrillarin antibody and Alexa 594-conjugated goat antimouse IgG antibody to reveal the nucleolus (No). The nucleus was stained using DAPI. Cyt, cytoplasm; bar, 10 micrometer.
