Fig. 3. Analysis of region between amino acids 126 and 136. The regions from amino acids 137 to 257, 109 to 257 and 70 to 125 in GFP-MCRS2 were deleted (GFP-d137/257, GFP-d109/257 and GFP-d70/125). The sequence between 126 and 136 was substituted for alanines (GFP-m126/129, GFP-m130/ 132 and GFPm133/ 136). At 18 h after transfection, proteins in the cell were stained with anti-fibrillarin antibody and Alexa 594-conjugated goat anti-mouse IgG antibody to reveal the nucleolus (No). The nucleus (N) was stained using DAPI. Bar, 10 micrometer.
