Fig. 4. Depletion of N-WASP affects kinetochore localization of CENP-A, CEPN-E, and Mad2. Control and N-WASP-deficient cells were stained for CENP-A, CENP-E, Mad2 (Red), and DNA (TOPRO3). Immunostaining of CENP-A was performed in nocodazole-treated prometaphase cells and 60 min after removal of nocodazole. Kinetochore localization of CENP-E and Mad2 were examined in nocodazoletreated prometaphase cells. CENP-A, CENP-E, and Mad2 signals were hardly detectable in N-WASP-deficient cells.
