Fig. 4. Gel-filtration chromatography of the total protein extracts from acg1 overexpressing HA:FLAG:FVE. (A) Chromatogram of the total protein extracts from acg1 overexpressing HA:FLAG:FVE. The total proteins from two-week-old Pro35S:HA:FLAG:FVE/acg1 (N1-11) plants were separated on a Sephacryl S-300 gel filtration column and fractionated at 1 ml. The eluted proteins were monitored at 280 nm. (B) Immunoblot analyses of each fraction received from gel-filtration chromatography. Equal volumes of each fraction were separated by 10% SDS-PAGE and subjected to immunoblot analysis with HA antibody (A). Each fraction was stained by Coomassie blue dye (B).
