Fig. 2. Effects of Wnt5a on collagen- or U46619-induced rat platelet aggregation. The degree of platelet aggregation was measured based on the level of light absorption. Rat PRP was stimulated by (A) collagen (0.7 μg/ml) with or without Wnt5a (50 or 100 ng) or (B) U46619 (a thromboxane A2 mimetic, 3 μM) with or without Wnt5a (50 or 100 ng). Collagen (0.5 μg/ml) was added 30 s prior to stimulation. The aggregation traces are representative of three separate experiments.