Fig. 4. Wortmannin blocks potentiation of platelet aggregation by U46619 and Wnt5a. PRP was stimulated with U46619 (3 μM) with or without Wnt5a (100 ng). Wortmannin (10 μM) or vehicle was added 30 s prior to stimulation. Collagen (0.5 μg/ml) was added 30 s prior to stimulation. The aggregation traces are representative of three separate experiments.