Fig. 2. Effect of docetaxel on the expression of apoptosis-related genes in AGS and AGS-EBV cells. Cells were treated with 30 nM docetaxel for 72 h. (A) Cell lysate (50 μg protein/lane) was analyzed by Western blot using anti-Bcl-2 (1:500), anti-Bax (1:2000), and anti-cleaved caspase- 3 (1:500) antibodies. Anti-β-actin antibody (1:2000) was used to check the loading amount. The expression ratio of Bcl-2, Bax, or caspase-3 relative to that of β-actin (B) and the expression ratio of Bcl- 2 to Bax (C) are also shown. The data are presented as mean ± SD of three independent experiments. (D) The level of Bcl- 2 mRNA expression was determined by real-time RT-PCR following treatment with 30 nM docetaxel for 72 h. Data are shown as mean ± SD of triplicate experiments.