Fig. 1. Phenotype of surface markers in CD34⁺ HSCs. FACS analysis depicting the immunophenotype of CD34⁺ cells that were obtained by magnetic column separation of human peripheral blood. (A) IgG1 isotype control staining. Cells were stained with anti-CD34-PE (B); anti-CD133-PE (C); anti-CD45-PE (D); or anti-CD73-PE (E) human antibodies. (F) Summary of the FACS analysis. Each column gives the mean ± SD (n = 3) of the percentage of the markerpositive cells of the total number of cells analyzed.