Fig. 4. Characteristics of TASK2-like K+ currents in CD34+ HSCs. (A) Representative traces of pHe- sensitive currents recorded by the ramp-like voltage change from -40 to 100 mV (see inset) in different pH solutions (pH 6.4; 7.4; and 8.4). Similar results were observed in 19 of 50 cells. (B) At -80 mV, inward currents were detected when extracellular Na+ was replaced with K+, which was inhibited by an acidic pHe of 6.4. (C) Representative traces of single-channel activity of the pHe-sensitive K+ current at negative voltages in symmetrical K+ conditions. The unitary amplitudes were plotted against the clamp voltages, and the slope conductance was 63 pS (n = 1).
