Fig. 5. Functional activities of TRP family currents in CD34⁺ HSCs. (A) Representative trace of TRPM2 current in CD34⁺ HSCs internally dialyzed with 100 μM ADPR. Similar results were observed in 6 out of 30 cells tested. Steplike inward currents increased spontaneously after establishing the whole cell configuration (holding voltage, -60 mV) and were completely abolished by replacing extracellular Na⁺ with NMDG⁺. (B) An original chart trace of membrane currents demonstrating the high temperature (HT, 42℃) activated current in CD34⁺ HSCs (left panel). The heatactivated inward current was largely inhibited by replacing extracellular Na⁺ with NMDG⁺. Representative currents were obtained with a ramp pulse (from -120 to 60 mV, held at -40 mV) shown in the left panel with the I-V relationship shown in the right panel. RT; room temperature (23℃). Similar results were observed in 32 out of 46 cells tested. (C) Bath application of OAG (100 μM) increased whole-cell currents of CD34⁺ HSCs (left panel). Representative I/V curves were obtained with a ramp pulse (from -100 to 60 mV, held at -60 mV) shown in the left panel with the I-V relationship shown in the right panel. Similar results were observed in 3 out of 6 cells tested.